domie2307 domie2307

24-01-2024
Biology

contestada

As an intern in a biology institute, I have a 17.3kbp plasmid that needs to be transformed into E. coli. However, I have tried multiple times and have had no or very few colonies on the LB plate. I am aware that large plasmids have a lower frequency of transformation. How can I improve the chances of successful transformation?

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